| 64 | Matia Cristiani (BAB) def. Natalia Leroy (WES-W) 6-2, 6-4 |
| 64 | Agnes Guggenheim (WES-W) def. Isabelle Gutierrez (COL-SAW) 6-0, 6-1 |
| 64 | Mariia Kornilova (WES-W) def. Heidi Cambron (SMITH) 6-1, 6-2 |
| 64 | Lara Afolayanka (WES-W) def. Rayna Li (MIT) 6-2, 6-0 |
| 64 | Leila Epstein (WES-W) def. Caroline Stuhlfaut (BOW) 6-2, 6-2 |
| 64 | Sarah Youngberg (WES-W) def. Zainab Patel (UMASS-B) 6-1, 6-0 |
| 32 | Elsie Van Wieren (MID) def. Agnes Guggenheim (WES-W) 6-2, 6-2 |
| 32 | Mariia Kornilova (WES-W) def. Waka Aida (COLBY) 6-3, 6-2 |
| 32 | Gusty Beveridge (MID) def. Lara Afolayanka (WES-W) 6-2, 7-5 |
| 32 | Leila Epstein (WES-W) def. Kamora Helton (NICHOLS) 6-2, 6-2 |
| 32 | Fiona Lee (MIT) def. Sarah Youngberg (WES-W) 6-2, 6-3 |
| 16 | Tina Liu (BAB) def. Mariia Kornilova (WES-W) 3-6, 6-1, 6-1 |
| 16 | Leila Epstein (WES-W) def. Marie Reyes (BRAND) 6-2, 3-6, 6-1 |
| QF | Fiona Lee (MIT) def. Leila Epstein (WES-W) 6-1, 4-6, 6-3 |
| B32 | Lucinda Gatsiounis (WES-W) def. Rachel Roth (AMH) 7-5, 7-5 |
| B32 | Kendall Smith (WES-W) def. Emma Bazemore (TRIN) 6-1, 6-0 |
| B16 | Nancy Zhang (BRAND) def. Lucinda Gatsiounis (WES-W) retired |
| B16 | Kendall Smith (WES-W) def. Maya Muhunthan (AMH) 6-2, 6-3 |
| BQF | Ren Barton (MID) def. Kendall Smith (WES-W) 6-4, 1-2, retired |
